Journal: bioRxiv

Article Title: Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer

doi: 10.1101/2023.02.06.527341

Figure Lengend Snippet: The bar graph gives the percent ALDH+, LRIG1+, CD166+, and LGR5+ cells in the HT29 CRC cell population as determined by flow cytometric analysis. Error bars represent standard error of the mean (n = 3–7 experimental replicates per marker).

Article Snippet: The following IgGs were included: mouse IgG1 – PE conjugate (5 uL for CD166; BD Biosciences #555749), purified sheep IgG (2 mg/mL for LRIG1 R&D Systems #5–001-A), and mouse IgG1-PE conjugate (10 uL for LGR5; R&D Systems #IC002P).

Techniques: Marker

Journal: bioRxiv

Article Title: Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer

doi: 10.1101/2023.02.06.527341

Figure Lengend Snippet: This figure gives results from FACS analysis of stained CRC cells using a combination of the SC marker pair ALDH & LGR5. Panel A gives a representative dot blot graph illustrating the proportion of cells in LGR5+/ALDH−, ALDH+/LGR5−, LGR5+/ALDH+, and LGR5−/ALDH− cell subpopulations. Panel B gives a bar graph showing the average percentage positive cells in each of the four different CSC subpopulations. Error bars represent standard error of the mean. Panel C gives a heatmap from NanoString profiling analysis showing differential expression of the top 50 miRNAs in the four different isolated CSC subpopulations (increased expression = green; decreased expression = red for each CSC marker). NanoString miRNA profiling. Panel D provides the same heatmap with the respective dendrogram. Panels C and D visually illustrate the patterns that are seen when a large set of miRNAs is surveyed and they are not meant to show details, which is why the vertical axes are not legible.

Article Snippet: The following IgGs were included: mouse IgG1 – PE conjugate (5 uL for CD166; BD Biosciences #555749), purified sheep IgG (2 mg/mL for LRIG1 R&D Systems #5–001-A), and mouse IgG1-PE conjugate (10 uL for LGR5; R&D Systems #IC002P).

Techniques: Staining, Marker, Dot Blot, Expressing, Isolation

Journal: bioRxiv

Article Title: Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer

doi: 10.1101/2023.02.06.527341

Figure Lengend Snippet: The table lists the top 10 miRNAs expressed in LGR5+/ALDH− cells. miRNAs were ranked according to p -value <0.05 for statistical significance. The asterisk depicts the original name for the miRNA that was abbreviated in the Table. The pie chart shows the functional classification identified by David analysis of the mRNAs predicted to be targeted by the miRNAs expressed in LGR5+/ALDH− cells. A summary of the functions of mRNAs predicted to be targeted by the miRNAs is given in .

Article Snippet: The following IgGs were included: mouse IgG1 – PE conjugate (5 uL for CD166; BD Biosciences #555749), purified sheep IgG (2 mg/mL for LRIG1 R&D Systems #5–001-A), and mouse IgG1-PE conjugate (10 uL for LGR5; R&D Systems #IC002P).

Techniques: Functional Assay

Journal: bioRxiv

Article Title: Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer

doi: 10.1101/2023.02.06.527341

Figure Lengend Snippet: The table lists the top 10 miRNAs expressed in ALDH+/LGR5− cells. miRNAs were ranked according to p -value <0.05 for statistical significance. The pie chart shows the functional classification identified by David analysis of the mRNAs predicted to be targeted by the miRNAs expressed in ALDH+/LGR5− cells. A summary of the functions of mRNAs predicted to be targeted by the miRNAs is given in .

Article Snippet: The following IgGs were included: mouse IgG1 – PE conjugate (5 uL for CD166; BD Biosciences #555749), purified sheep IgG (2 mg/mL for LRIG1 R&D Systems #5–001-A), and mouse IgG1-PE conjugate (10 uL for LGR5; R&D Systems #IC002P).

Techniques: Functional Assay

Journal: bioRxiv

Article Title: Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer

doi: 10.1101/2023.02.06.527341

Figure Lengend Snippet: The following 25 mRNAs are predicted to be targeted by miRNAs that are selectively expressed in LGR5+ cells (not expressed in ALDH+ cells): ADH1B, ADH4, ADH5, ADH6, ADH7, ALDH16A1, ALDH1A3, ALDH1L1, ALDH2, ALDH3A1, ALDH3A2, ALDH3B2, ALDH4A1, ALDH5A1, ALDH7A1, ALDH9A1, CRABP2, RBP2, RBP4, RBP5, RDH11, RDH13, RDH5, RDH8, RXRB .

Article Snippet: The following IgGs were included: mouse IgG1 – PE conjugate (5 uL for CD166; BD Biosciences #555749), purified sheep IgG (2 mg/mL for LRIG1 R&D Systems #5–001-A), and mouse IgG1-PE conjugate (10 uL for LGR5; R&D Systems #IC002P).

Techniques:

Journal: bioRxiv

Article Title: Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer

doi: 10.1101/2023.02.06.527341

Figure Lengend Snippet: MicroRNA expression patterns in ALDH+ cells and LGR5+ cells in CSC sub-populations isolated from different FACS sorting experiments

Article Snippet: The following IgGs were included: mouse IgG1 – PE conjugate (5 uL for CD166; BD Biosciences #555749), purified sheep IgG (2 mg/mL for LRIG1 R&D Systems #5–001-A), and mouse IgG1-PE conjugate (10 uL for LGR5; R&D Systems #IC002P).

Techniques: Expressing, Isolation

Journal: Frontiers in Immunology

Article Title: Metabolic Reprograming of Cystic Fibrosis Macrophages via the IRE1α Arm of the Unfolded Protein Response Results in Exacerbated Inflammation

doi: 10.3389/fimmu.2019.01789

Figure Lengend Snippet: Table of Reagents

Article Snippet: Normal mouse IgG1 PE (Isotype) , Santa Cruz , Cat# sc-2866; RRID: AB_737219.

Techniques: Control, Purification, Recombinant, Isolation, SYBR Green Assay, Protease Inhibitor, Western Blot, Reverse Transcription, Enzyme-linked Immunosorbent Assay, Conjugation Assay, Bicinchoninic Acid Protein Assay, Software